NHA LABORATORY

Analytical Capabilities

The available methodologies at the Laboratory vary from sensitive immuno-diagnostic methods to drug specific mass spectrometric methods, depending on the drug class and required sensitivity (often low part per billion sensitivity).  In line with modern international advances, solid phase extraction (dual phase, synthetic phase and diatomaceous earth) and extensive use of mass spectrometry (GC/MSn and LC/MSn) as detection methodology form an integral part of analysis at the Laboratory.  Methodologies which are in place and which have been developed are designed for robustness and high throughput analysis.  This ensures short reporting times.  A 4-probe automated solid phase extraction system, 4 LC/MS systems and 6 GC/MS systems ensure that deadlines can be met even should high workloads and large contracts be encountered.

Extraction and detection techniques

The following extraction, chromatographic and detection techniques and instrumentation are available and being employed in the Laboratory for contract analysis:
  • Liquid/liquid extraction
  • Solid phase extraction
  • Automated solid phase extraction (4-probe)
  • HPLC/UV (DAD detection)
  • GC/MS (Quadrupole)
  • GC/MSn (Ion Trap)
  • LC/MSn (Ion Trap)
  • LC/MS3 (Hybrid Triple Quadrupole Linear Ion Trap)
  • LC/MS/MS (Triple Quadrupole)
  • Radio-immunoassays (beta and gamma radiation counting)
  • Enzyme linked immunoassays (ELISA)

Drug classes routinely screened

TThe following drug classes are or can be screened for at the Laboratory as part of horseracing and animal sports contract analysis:
  • Anabolic steroids (injectable and oral)
  • Analgesics
  • Antidepressants
  • Antihistamines
  • Barbiturates
  • Beta-agonists
  • Beta-blockers
  • Bronchodilators
  • Corticosteroids (injectable and nebulised)
  • Dimethylsulfoxide (DMSO)
  • Diuretics
  • Local anaesthetics
  • Muscle relaxants
  • Narcotics
  • Non-steroidal anti-inflammatory drugs (NSAID's)
  • Sedatives
  • Stimulants
  • Tranquilisers
  • Protein hormones (Growth Hormone (GH) and Erythropoietin (EPO))
and other less prominent drug classes.